Hey Mark,
The one we have been using is the BD one - clone Q21-559 (we used AF488 because it fit in our panel). https://www.bdbiosciences.com/content/bdb/paths/generate-tds-document.us.563621.pdf
The key is also the transcription factor buffer set from BD. It does not seem to affect the surface expression of chemokines in our data.
I hope that helps.
Thanks,
Nikko
Category:
Materials:
562574 Transcription Factor Buffer Set 100 tests (none)
562725 Transcription Factor Buffer Set 25 tests (none)
Alexa Fluor® 488 Mouse Anti-Human RORγt
Product Information
Material Number: 563621
Alternate Name: RORgt; ROR gamma t; RORC; NR1F3; RORG; RZRG; TOR; RZR-GAMMA
Size: 50 tests
Vol. per Test: 5 μl
Clone: Q21-559
562725 Transcription Factor Buffer Set 25 tests (none)
Alexa Fluor® 488 Mouse Anti-Human RORγt
Product Information
Material Number: 563621
Alternate Name: RORgt; ROR gamma t; RORC; NR1F3; RORG; RZRG; TOR; RZR-GAMMA
Size: 50 tests
Vol. per Test: 5 μl
Clone: Q21-559