Mark Ansel, PhD

Professor
Microbiology & Immunology
+1 415 476-5368

MicroRNAs, transcription factors, and epigenetic regulation shape the gene expression programs that determine cell identity and function. The Ansel lab studies how these molecular mechanisms work together to control lymphocyte development, differentiation, and function in immunity.

We use in vitro cell differentiation systems, mouse genetics, disease models, and high dimensional cellular and molecular analyses in human biospecimens to unravel the regulatory networks that underlie immunity and immune pathology, especially allergy and asthma.

Lymphocyte lineage decisions and the deployment of their effector functions are critical for the development of protective immunity against a great diversity of pathogens. However, improper or exaggerated responses underlie the pathogenesis of autoimmune diseases, chronic inflammation, allergy, and asthma. Our primary experimental system is the differentiation of helper T cells, the central coordinators of adaptive immune responses. Upon immune activation, naïve CD4+ T cells can differentiate into several different helper T cell effectors subtypes (e.g. Th1, Th2, Th17, iTreg, Tfh, etc.). These lineages are defined by their characteristic gene expression programs and mediate distinct immune functions. These gene expression programs are controlled by external factors that derive from other cells or the environment, signaling-induced and lineage-specific transcription factors, epigenetic regulation of transcriptional responses, and posttranscriptional mechanisms, including RNA-binding proteins and miRNAs. The depth of our knowledge about the networks that control helper T cells makes them an attractive model for studying basic mechanisms of gene regulation.

Active projects in the laboratory mostly focus on RNA regulation of immune cell programming. We study how individual miRNA families regulate lymphocyte differentiation and immune function, and the regulation of the miRNA pathway itself during immune responses. Naive CD4+ T cells that cannot produce any miRNAs exhibit reduced cell division and survival in response to immune stimuli. Surprisingly, they also undergo rapid unrestrained differentiation into effector cells. We have developed a screening technology that allows us to rapidly determine which specific miRNAs regulate each of these T cell behaviors, and pipelines for determining miRNA expression patterns in very small clinical samples (such as sorted T cell subsets from the airways of human asthmatic subjects, serum, sputum, and other sources of extracellular miRNAs, etc.). In addition, we discovered that T cells rapidly reset their small RNA repertoire upon activation. This process involves ubiquitination and degradation of Argonaute proteins and the release of RNAs in extracellular vesicles, but the signaling mechanisms and the fate of associated RNAs remains unknown. Activation-induced changes in regulatory RNA expression affects T cell differentiation and the development of immune effector functions.

The major research goals of our laboratory are:

1) To define the molecular mechanisms that control miRNA homeostasis and extracellular release by lymphocytes, and determine how the miRNA repertoire is so dramatically remodeled during activation.

2) To characterize the function of RBPs and individual miRNAs that regulate T cell differentiation and immune effector functions.

3) To determine how the expression and function of miRNAs contributes to the pathogenic properties of T cells and other immune cells in human asthma.

Publications: 

The lncRNA Malat1 inhibits miR-15/16 to enhance cytotoxic T cell activation and memory cell formation.

eLife

Wheeler BD, Gagnon JD, Zhu WS, Muñoz-Sandoval P, Wong SK, Simeonov DS, Li Z, DeBarge R, Spitzer MH, Marson A, Ansel KM

GCLiPP: global crosslinking and protein purification method for constructing high-resolution occupancy maps for RNA binding proteins.

Genome biology

Zhu WS, Litterman AJ, Sekhon HS, Kageyama R, Arce MM, Taylor KE, Zhao W, Criswell LA, Zaitlen N, Erle DJ, Ansel KM

An essential role for miR-15/16 in Treg suppression and restriction of proliferation.

Cell reports

Johansson K, Gagnon JD, Zhou SK, Fassett MS, Schroeder AW, Kageyama R, Bautista RA, Pham H, Woodruff PG, Ansel KM

IL-31-dependent neurogenic inflammation restrains cutaneous type 2 immune response in allergic dermatitis.

Science immunology

Fassett MS, Braz JM, Castellanos CA, Salvatierra JJ, Sadeghi M, Yu X, Schroeder AW, Caston J, Munoz-Sandoval P, Roy S, Lazarevsky S, Mar DJ, Zhou CJ, Shin JS, Basbaum AI, Ansel KM

RNA circuits and RNA-binding proteins in T cells.

Trends in immunology

Zhu WS, Wheeler BD, Ansel KM

The miR-23-27-24 clusters drive lipid-associated macrophage proliferation in obese adipose tissue.

Cell reports

Sprenkle NT, Winn NC, Bunn KE, Zhao Y, Park DJ, Giese BG, Karijolich JJ, Ansel KM, Serezani CH, Hasty AH, Pua HH

The lncRNA Malat1 Inhibits miR-15/16 to Enhance Cytotoxic T Cell Activation and Memory Cell Formation.

bioRxiv : the preprint server for biology

Wheeler BD, Gagnon JD, Zhu WS, Muñoz-Sandoval P, Wong SK, Simeonov DS, Li Z, Debarge R, Spitzer M, Marson A, Ansel KM

Null and missense mutations of ERI1 cause a recessive phenotypic dichotomy in humans.

American journal of human genetics

Guo L, Salian S, Xue JY, Rath N, Rousseau J, Kim H, Ehresmann S, Moosa S, Nakagawa N, Kuroda H, Clayton-Smith J, Wang J, Wang Z, Banka S, Jackson A, Zhang YM, Wei ZJ, Hüning I, Brunet T, Ohashi H, Thomas MF, Bupp C, Miyake N, Matsumoto N, Mendoza-Londono R, Costain G, Hahn G, Di Donato N, Yigit G, Yamada T, Nishimura G, Ansel KM, Wollnik B, Hrabe de Angelis M, Mégarbané A, Rosenfeld JA, Heissmeyer V, Ikegawa S, Campeau PM

Stimulation of ectopically expressed muscarinic receptors induces IFN-γ but suppresses IL-2 production by inhibiting activation of pAKT pathways in primary T cells.

Proceedings of the National Academy of Sciences of the United States of America

Nguyen TTT, Lu W, Zhu WS, Ansel KM, Liang HE, Weiss A

An essential role for miR-15/16 in Treg suppression and restriction of proliferation.

bioRxiv : the preprint server for biology

Johansson K, Gagnon JD, Zhou S, Fassett MS, Schroeder AW, Kageyama R, Bautista RA, Pham H, Woodruff PG, Ansel KM

Forced expression of the non-coding RNA miR-17~92 restores activation and function in CD28-deficient CD4+ T�cells.

iScience

D?lz M, Hasiuk M, Gagnon JD, Kornete M, Marone R, Bantug G, Kageyama R, Hess C, Ansel KM, Seyres D, Roux J, Jeker LT

Innate type 2 immunity controls hair follicle commensalism by Demodex mites.

Immunity

Ricardo-Gonzalez RR, Kotas ME, O'Leary CE, Singh K, Damsky W, Liao C, Arouge E, Tenvooren I, Marquez DM, Schroeder AW, Cohen JN, Fassett MS, Lee J, Daniel SG, Bittinger K, Díaz RE, Fraser JS, Ali N, Ansel KM, Spitzer MH, Liang HE, Locksley RM

Mass cytometry reveals a conserved immune trajectory of recovery in hospitalized COVID-19 patients.

Immunity

Burnett CE, Okholm TLH, Tenvooren I, Marquez DM, Tamaki S, Munoz Sandoval P, Willmore A, UCSF COMET Consortium, Hendrickson CM, Kangelaris KN, Langelier CR, Krummel MF, Woodruff PG, Calfee CS, Erle DJ, Ansel KM, Spitzer MH

MicroRNA-29a attenuates CD8 T cell exhaustion and induces memory-like CD8 T cells during chronic infection.

Proceedings of the National Academy of Sciences of the United States of America

Stelekati E, Cai Z, Manne S, Chen Z, Beltra JC, Buchness LA, Leng X, Ristin S, Nzingha K, Ekshyyan V, Niavi C, Abdel-Hakeem MS, Ali MA, Drury S, Lau CW, Gao Z, Ban Y, Zhou SK, Ansel KM, Kurachi M, Jordan MS, Villarino AV, Ngiow SF, Wherry EJ

Obesity alters pathology and treatment response in inflammatory disease.

Nature

Bapat SP, Whitty C, Mowery CT, Liang Y, Yoo A, Jiang Z, Peters MC, Zhang LJ, Vogel I, Zhou C, Nguyen VQ, Li Z, Chang C, Zhu WS, Hastie AT, He H, Ren X, Qiu W, Gayer SG, Liu C, Choi EJ, Fassett M, Cohen JN, Sturgill JL, Crotty Alexander LE, Suh JM, Liddle C, Atkins AR, Yu RT, Downes M, Liu S, Nikolajczyk BS, Lee IK, Guttman-Yassky E, Ansel KM, Woodruff PG, Fahy JV, Sheppard D, Gallo RL, Ye CJ, Evans RM, Zheng Y, Marson A

A conserved immune trajectory of recovery in hospitalized COVID-19 patients.

bioRxiv : the preprint server for biology

Burnett CE, Hauge Okholm TL, Tenvooren I, Marquez DM, Tamaki S, Sandoval PM, UCSF COMET Consortium, Calfee CS, Hendrickson CM, Kangelaris KN, Langelier CR, Krummel MF, Woodruff PG, Erle DJ, Ansel KM, Spitzer MH

Impaired antibacterial immune signaling and changes in the lung microbiome precede secondary bacterial pneumonia in COVID-19.

medRxiv : the preprint server for health sciences

Tsitsiklis A, Zha BS, Byrne A, Devoe C, Levan S, Rackaityte E, Sunshine S, Mick E, Ghale R, Jauregui A, Sarma A, Neff N, Serpa PH, Deiss TJ, Kistler A, Carrillo S, Ansel KM, Leligdowicz A, Christenson S, Jones N, Wu B, Darmanis S, Matthay MA, Lynch SV, DeRisi JL, COMET Consortium+ , Hendrickson CM, Kangelaris KN, Krummel MF, Woodruff PG, Erle DJ, Rosenberg O, Calfee CS, Langelier CR

RNA regulation in immunity.

Immunological reviews

Pua H, Ansel KM

MicroRNA-directed pathway discovery elucidates an miR-221/222-mediated regulatory circuit in class switch recombination.

The Journal of experimental medicine

Wigton EJ, Mikami Y, McMonigle RJ, Castellanos CA, Wade-Vallance AK, Zhou SK, Kageyama R, Litterman A, Roy S, Kitamura D, Dykhuizen EC, Allen CDC, Hu H, O'Shea JJ, Ansel KM

PICS2: Next-generation fine mapping via probabilistic identification of causal SNPs.

Bioinformatics (Oxford, England)

Taylor KE, Ansel KM, Marson A, Criswell LA, Farh KK

Regulation of airway immunity by epithelial miRNAs.

Immunological reviews

Johansson K, Woodruff PG, Ansel KM

Tracheal aspirate RNA sequencing identifies distinct immunological features of COVID-19 ARDS.

Nature communications

Sarma A, Christenson SA, Byrne A, Mick E, Pisco AO, DeVoe C, Deiss T, Ghale R, Zha BS, Tsitsiklis A, Jauregui A, Moazed F, Detweiler AM, Spottiswoode N, Sinha P, Neff N, Tan M, Serpa PH, Willmore A, Ansel KM, Wilson JG, Leligdowicz A, Siegel ER, Sirota M, DeRisi JL, Matthay MA, COMET Consortium , Hendrickson CM, Kangelaris KN, Krummel MF, Woodruff PG, Erle DJ, Calfee CS, Langelier CR

Impaired immune signaling and changes in the lung microbiome precede secondary bacterial pneumonia in COVID-19.

Research square

Tsitsiklis A, Zha B, Byrne A, DeVoe C, Levan S, Rackaityte E, Sunshine S, Mick E, Ghale R, Jauregui A, Neff N, Sarma A, Serpa P, Deiss T, Kistler A, Carrillo S, Ansel KM, Leligdowicz A, Christenson S, Jones N, Wu B, Darmanis S, Matthay M, Lynch S, DeRisi J, Consortium C, Hendrickson C, Kangelaris K, Krummel M, Woodruff P, Erle D, Rosenberg O, Calfee C, Langelier C

Epithelial miR-141 regulates IL-13-induced airway mucus production.

JCI insight

Siddiqui S, Johansson K, Joo A, Bonser LR, Koh KD, Le Tonqueze O, Bolourchi S, Bautista RA, Zlock L, Roth TL, Marson A, Bhakta NR, Ansel KM, Finkbeiner WE, Erle DJ, Woodruff PG

COVID-19 ARDS is characterized by a dysregulated host response that differs from cytokine storm and is modified by dexamethasone.

Research square

Sarma A, Christenson S, Mick E, Deiss T, DeVoe C, Pisco A, Ghale R, Jauregui A, Byrne A, Moazed F, Spottiswoode N, Sinha P, Zha B, Neff N, Tan M, Serpa PH, Ansel KM, Wilson J, Leligdowicz A, Seigel E, Sirota M, DeRisi J, Matthay M, Consortium C, Hendrickson C, Kangelaris K, Krummel M, Woodruff P, Erle D, Calfee C, Langelier C

miR-29 Sustains B Cell Survival and Controls Terminal Differentiation via Regulation of PI3K Signaling.

Cell reports

Hines MJ, Coffre M, Mudianto T, Panduro M, Wigton EJ, Tegla C, Osorio-Vasquez V, Kageyama R, Benhamou D, Perez O, Bajwa S, McManus MT, Ansel KM, Melamed D, Koralov SB

Author Correction: Profiling immunoglobulin repertoires across multiple human tissues using RNA sequencing.

Nature communications

Mandric I, Rotman J, Yang HT, Strauli N, Montoya DJ, Van Der Wey W, Ronas JR, Statz B, Yao D, Petrova V, Zelikovsky A, Spreafico R, Shifman S, Zaitlen N, Rossetti M, Ansel KM, Eskin E, Mangul S

An Anti-Siglec-8 Antibody Depletes Sputum Eosinophils from Asthmatic Subjects and Inhibits Lung Mast Cells.

Clinical and experimental allergy : journal of the British Society for Allergy and Clinical Immunology

Kerr SC, Gonzalez JR, Schanin J, Peters MC, Lambrecht BN, Brock EC, Charbit A, Ansel KM, Youngblood BA, Fahy JV

Profiling immunoglobulin repertoires across multiple human tissues using RNA sequencing.

Nature communications

Mandric I, Rotman J, Yang HT, Strauli N, Montoya DJ, Van Der Wey W, Ronas JR, Statz B, Yao D, Petrova V, Zelikovsky A, Spreafico R, Shifman S, Zaitlen N, Rossetti M, Ansel KM, Eskin E, Mangul S

Distinct associations of sputum and oral microbiota with atopic, immunologic, and clinical features in mild asthma.

The Journal of allergy and clinical immunology

Durack J, Christian LS, Nariya S, Gonzalez J, Bhakta NR, Ansel KM, Beigelman A, Castro M, Dyer AM, Israel E, Kraft M, Martin RJ, Mauger DT, Peters SP, Rosenberg SR, Sorkness CA, Wechsler ME, Wenzel SE, White SR, Lynch SV, Boushey HA, Huang YJ

Universal Principled Review: A Community-Driven Method to Improve Peer Review.

Cell

Krummel M, Blish C, Kuhns M, Cadwell K, Oberst A, Goldrath A, Ansel KM, Chi H, O'Connell R, Wherry EJ, Pepper M

RNA Binding Protein CELF2 Regulates Signal-Induced Alternative Polyadenylation by Competing with Enhancers of the Polyadenylation Machinery.

Cell reports

Chatrikhi R, Mallory MJ, Gazzara MR, Agosto LM, Zhu WS, Litterman AJ, Ansel KM, Lynch KW

MicroRNA regulation of CD8+ T cell responses.

Non-coding RNA investigation

Gagnon JD, Ansel KM

miR-15/16 Restrain Memory T Cell Differentiation, Cell Cycle, and Survival.

Cell reports

Gagnon JD, Kageyama R, Shehata HM, Fassett MS, Mar DJ, Wigton EJ, Johansson K, Litterman AJ, Odorizzi P, Simeonov D, Laidlaw BJ, Panduro M, Patel S, Jeker LT, Feeney ME, McManus MT, Marson A, Matloubian M, Sanjabi S, Ansel KM

A massively parallel 3' UTR reporter assay reveals relationships between nucleotide content, sequence conservation, and mRNA destabilization.

Genome research

Litterman AJ, Kageyama R, Le Tonqueze O, Zhao W, Gagnon JD, Goodarzi H, Erle DJ, Ansel KM

Increased Hematopoietic Extracellular RNAs and Vesicles in the Lung during Allergic Airway Responses.

Cell reports

Pua HH, Happ HC, Gray CJ, Mar DJ, Chiou NT, Hesse LE, Ansel KM

Bacterial biogeography of adult airways in atopic asthma.

Microbiome

Durack J, Huang YJ, Nariya S, Christian LS, Mark Ansel K, Beigelman A, Castro M, Dyer AM, Israel E, Kraft M, Martin RJ, Mauger DT, Rosenberg SR, King TS, White SR, Denlinger LC, Holguin F, Lazarus SC, Lugogo N, Peters SP, Smith LJ, Wechsler ME, Lynch SV, Boushey HA

Identification of Functionally Relevant microRNAs in the Regulation of Allergic Inflammation.

Methods in molecular biology (Clifton, N.J.)

Fassett MS, Pua HH, Simpson LJ, Steiner DF, Mark Ansel K

MicroRNA regulation of type 2 innate lymphoid cell homeostasis and function in allergic inflammation.

The Journal of experimental medicine

Singh PB, Pua HH, Happ HC, Schneider C, von Moltke J, Locksley RM, Baumjohann D, Ansel KM

Discovery of stimulation-responsive immune enhancers with CRISPR activation.

Nature

Simeonov DR, Gowen BG, Boontanrart M, Roth TL, Gagnon JD, Mumbach MR, Satpathy AT, Lee Y, Bray NL, Chan AY, Lituiev DS, Nguyen ML, Gate RE, Subramaniam M, Li Z, Woo JM, Mitros T, Ray GJ, Curie GL, Naddaf N, Chu JS, Ma H, Boyer E, Van Gool F, Huang H, Liu R, Tobin VR, Schumann K, Daly MJ, Farh KK, Ansel KM, Ye CJ, Greenleaf WJ, Anderson MS, Bluestone JA, Chang HY, Corn JE, Marson A

A Distinct Inhibitory Function for miR-18a in Th17 Cell Differentiation.

Journal of immunology (Baltimore, Md. : 1950)

Montoya MM, Maul J, Singh PB, Pua HH, Dahlström F, Wu N, Huang X, Ansel KM, Baumjohann D

Small RNA Transfection in Primary Human Th17 Cells by Next Generation Electroporation.

Journal of visualized experiments : JoVE

Montoya MM, Ansel KM

Alternative splicing of interleukin-33 and type 2 inflammation in asthma.

Proceedings of the National Academy of Sciences of the United States of America

Gordon ED, Simpson LJ, Rios CL, Ringel L, Lachowicz-Scroggins ME, Peters MC, Wesolowska-Andersen A, Gonzalez JR, MacLeod HJ, Christian LS, Yuan S, Barry L, Woodruff PG, Ansel KM, Nocka K, Seibold MA, Fahy JV

IFN-?-Producing T-Helper 17.1 Cells Are Increased in Sarcoidosis and Are More Prevalent than T-Helper Type 1 Cells.

American journal of respiratory and critical care medicine

Ramstein J, Broos CE, Simpson LJ, Ansel KM, Sun SA, Ho ME, Woodruff PG, Bhakta NR, Christian L, Nguyen CP, Antalek BJ, Benn BS, Hendriks RW, van den Blink B, Kool M, Koth LL

MicroRNAs 24 and 27 Suppress Allergic Inflammation and Target a Network of Regulators of T Helper 2 Cell-Associated Cytokine Production.

Immunity

Pua HH, Steiner DF, Patel S, Gonzalez JR, Ortiz-Carpena JF, Kageyama R, Chiou NT, Gallman A, de Kouchkovsky D, Jeker LT, McManus MT, Erle DJ, Ansel KM

Biogenesis, delivery, and function of extracellular RNA.

Journal of extracellular vesicles

Patton JG, Franklin JL, Weaver AM, Vickers K, Zhang B, Coffey RJ, Ansel KM, Blelloch R, Goga A, Huang B, L'Etoille N, Raffai RL, Lai CP, Krichevsky AM, Mateescu B, Greiner VJ, Hunter C, Voinnet O, McManus MT

MicroRNA regulation of allergic inflammation and asthma.

Current opinion in immunology

Pua HH, Ansel KM

mir-181a-1/b-1 Modulates Tolerance through Opposing Activities in Selection and Peripheral T Cell Function.

Journal of immunology (Baltimore, Md. : 1950)

Schaffert SA, Loh C, Wang S, Arnold CP, Axtell RC, Newell EW, Nolan G, Ansel KM, Davis MM, Steinman L, Chen CZ

MicroRNA regulation of lymphocyte tolerance and autoimmunity.

The Journal of clinical investigation

Simpson LJ, Ansel KM

Self-enforcing feedback activation between BCL6 and pre-B cell receptor signaling defines a distinct subtype of acute lymphoblastic leukemia.

Cancer cell

Geng H, Hurtz C, Lenz KB, Chen Z, Baumjohann D, Thompson S, Goloviznina NA, Chen WY, Huan J, LaTocha D, Ballabio E, Xiao G, Lee JW, Deucher A, Qi Z, Park E, Huang C, Nahar R, Kweon SM, Shojaee S, Chan LN, Yu J, Kornblau SM, Bijl JJ, Ye BH, Ansel KM, Paietta E, Melnick A, Hunger SP, Kurre P, Tyner JW, Loh ML, Roeder RG, Druker BJ, Burger JA, Milne TA, Chang BH, Müschen M

Induced miR-99a expression represses Mtor cooperatively with miR-150 to promote regulatory T-cell differentiation.

The EMBO journal

Warth SC, Hoefig KP, Hiekel A, Schallenberg S, Jovanovic K, Klein L, Kretschmer K, Ansel KM, Heissmeyer V

The transcription factor NFAT promotes exhaustion of activated CD8? T cells.

Immunity

Martinez GJ, Pereira RM, Äijö T, Kim EY, Marangoni F, Pipkin ME, Togher S, Heissmeyer V, Zhang YC, Crotty S, Lamperti ED, Ansel KM, Mempel TR, Lähdesmäki H, Hogan PG, Rao A

Tracking early T follicular helper cell differentiation in vivo.

Methods in molecular biology (Clifton, N.J.)

Baumjohann D, Ansel KM

Editorial overview: Allergy and hypersensitivity.

Current opinion in immunology

Sperling AI, Ansel KM

A microRNA upregulated in asthma airway T cells promotes TH2 cytokine production.

Nature immunology

Simpson LJ, Patel S, Bhakta NR, Choy DF, Brightbill HD, Ren X, Wang Y, Pua HH, Baumjohann D, Montoya MM, Panduro M, Remedios KA, Huang X, Fahy JV, Arron JR, Woodruff PG, Ansel KM

Epigenomic analysis of primary human T cells reveals enhancers associated with TH2 memory cell differentiation and asthma susceptibility.

Nature immunology

Seumois G, Chavez L, Gerasimova A, Lienhard M, Omran N, Kalinke L, Vedanayagam M, Ganesan AP, Chawla A, Djukanovic R, Ansel KM, Peters B, Rao A, Vijayanand P

Eri1: a conserved enzyme at the crossroads of multiple RNA-processing pathways.

Trends in genetics : TIG

Thomas MF, L'Etoile ND, Ansel KM

MicroRNA regulation of the germinal center response.

Current opinion in immunology

Baumjohann D, Ansel KM

Comparative transcriptional and functional profiling defines conserved programs of intestinal DC differentiation in humans and mice.

Nature immunology

Watchmaker PB, Lahl K, Lee M, Baumjohann D, Morton J, Kim SJ, Zeng R, Dent A, Ansel KM, Diamond B, Hadeiba H, Butcher EC

The microRNA cluster miR-17~92 promotes TFH cell differentiation and represses subset-inappropriate gene expression.

Nature immunology

Baumjohann D, Kageyama R, Clingan JM, Morar MM, Patel S, de Kouchkovsky D, Bannard O, Bluestone JA, Matloubian M, Ansel KM, Jeker LT

RNA regulation of the immune system.

Immunological reviews

Ansel KM

Regulation of miRNA biogenesis and turnover in the immune system.

Immunological reviews

Bronevetsky Y, Ansel KM

Persistent antigen and germinal center B cells sustain T follicular helper cell responses and phenotype.

Immunity

Baumjohann D, Preite S, Reboldi A, Ronchi F, Ansel KM, Lanzavecchia A, Sallusto F

T cell activation induces proteasomal degradation of Argonaute and rapid remodeling of the microRNA repertoire.

The Journal of experimental medicine

Bronevetsky Y, Villarino AV, Eisley CJ, Barbeau R, Barczak AJ, Heinz GA, Kremmer E, Heissmeyer V, McManus MT, Erle DJ, Rao A, Ansel KM

Eri1 degrades the stem-loop of oligouridylated histone mRNAs to induce replication-dependent decay.

Nature structural & molecular biology

Hoefig KP, Rath N, Heinz GA, Wolf C, Dameris J, Schepers A, Kremmer E, Ansel KM, Heissmeyer V

An integrated nano-scale approach to profile miRNAs in limited clinical samples.

American journal of clinical and experimental immunology

Seumois G, Vijayanand P, Eisley CJ, Omran N, Kalinke L, North M, Ganesan AP, Simpson LJ, Hunkapiller N, Moltzahn F, Woodruff PG, Fahy JV, Erle DJ, Djukanovic R, Blelloch R, Ansel KM

Airway epithelial miRNA expression is altered in asthma.

American journal of respiratory and critical care medicine

Solberg OD, Ostrin EJ, Love MI, Peng JC, Bhakta NR, Hou L, Nguyen C, Solon M, Nguyen C, Barczak AJ, Zlock LT, Blagev DP, Finkbeiner WE, Ansel KM, Arron JR, Erle DJ, Woodruff PG

Eri1 regulates microRNA homeostasis and mouse lymphocyte development and antiviral function.

Blood

Thomas MF, Abdul-Wajid S, Panduro M, Babiarz JE, Rajaram M, Woodruff P, Lanier LL, Heissmeyer V, Ansel KM

Interleukin-4 production by follicular helper T cells requires the conserved Il4 enhancer hypersensitivity site V.

Immunity

Vijayanand P, Seumois G, Simpson LJ, Abdul-Wajid S, Baumjohann D, Panduro M, Huang X, Interlandi J, Djuretic IM, Brown DR, Sharpe AH, Rao A, Ansel KM

MicroRNA-29 regulates T-box transcription factors and interferon-? production in helper T cells.

Immunity

Steiner DF, Thomas MF, Hu JK, Yang Z, Babiarz JE, Allen CD, Matloubian M, Blelloch R, Ansel KM

Cutting Edge: Distinct waves of BCL6 expression during T follicular helper cell development.

Journal of immunology (Baltimore, Md. : 1950)

Baumjohann D, Okada T, Ansel KM

Induction and maintenance of IL-4 expression are regulated differently by the 3' enhancer in CD4 T cells.

Journal of immunology (Baltimore, Md. : 1950)

Sofi MH, Qiao Y, Ansel KM, Kubo M, Chang CH

Six RNA viruses and forty-one hosts: viral small RNAs and modulation of small RNA repertoires in vertebrate and invertebrate systems.

PLoS pathogens

Parameswaran P, Sklan E, Wilkins C, Burgon T, Samuel MA, Lu R, Ansel KM, Heissmeyer V, Einav S, Jackson W, Doukas T, Paranjape S, Polacek C, dos Santos FB, Jalili R, Babrzadeh F, Gharizadeh B, Grimm D, Kay M, Koike S, Sarnow P, Ronaghi M, Ding SW, Harris E, Chow M, Diamond MS, Kirkegaard K, Glenn JS, Fire AZ

Construction of small RNA cDNA libraries for deep sequencing.

Methods in molecular biology (Clifton, N.J.)

Thomas MF, Ansel KM

Mouse Eri1 interacts with the ribosome and catalyzes 5.8S rRNA processing.

Nature structural & molecular biology

Ansel KM, Pastor WA, Rath N, Lapan AD, Glasmacher E, Wolf C, Smith LC, Papadopoulou N, Lamperti ED, Tahiliani M, Ellwart JW, Shi Y, Kremmer E, Rao A, Heissmeyer V

Role of CXCR5 and CCR7 in follicular Th cell positioning and appearance of a programmed cell death gene-1high germinal center-associated subpopulation.

Journal of immunology (Baltimore, Md. : 1950)

Haynes NM, Allen CD, Lesley R, Ansel KM, Killeen N, Cyster JG

The NFAT1 transcription factor is a repressor of cyclin A2 gene expression.

Cell cycle (Georgetown, Tex.)

Carvalho LD, Teixeira LK, Carrossini N, Caldeira AT, Ansel KM, Rao A, Viola JP

Regulation of the germinal center response by microRNA-155.

Science (New York, N.Y.)

Thai TH, Calado DP, Casola S, Ansel KM, Xiao C, Xue Y, Murphy A, Frendewey D, Valenzuela D, Kutok JL, Schmidt-Supprian M, Rajewsky N, Yancopoulos G, Rao A, Rajewsky K

Transcription factors T-bet and Runx3 cooperate to activate Ifng and silence Il4 in T helper type 1 cells.

Nature immunology

Djuretic IM, Levanon D, Negreanu V, Groner Y, Rao A, Ansel KM

Regulation of Th2 differentiation and Il4 locus accessibility.

Annual review of immunology

Ansel KM, Djuretic I, Tanasa B, Rao A

MicroRNA profiling of the murine hematopoietic system.

Genome biology

Monticelli S, Ansel KM, Xiao C, Socci ND, Krichevsky AM, Thai TH, Rajewsky N, Marks DS, Sander C, Rajewsky K, Rao A, Kosik KS

Aberrant T cell differentiation in the absence of Dicer.

The Journal of experimental medicine

Muljo SA, Ansel KM, Kanellopoulou C, Livingston DM, Rao A, Rajewsky K

A plague of autoantibodies.

Nature immunology

Heissmeyer V, Ansel KM, Rao A

Intrinsic lymphotoxin-beta receptor requirement for homeostasis of lymphoid tissue dendritic cells.

Immunity

Kabashima K, Banks TA, Ansel KM, Lu TT, Ware CF, Cyster JG

Deletion of a conserved Il4 silencer impairs T helper type 1-mediated immunity.

Nature immunology

Ansel KM, Greenwald RJ, Agarwal S, Bassing CH, Monticelli S, Interlandi J, Djuretic IM, Lee DU, Sharpe AH, Alt FW, Rao A

Germinal center dark and light zone organization is mediated by CXCR4 and CXCR5.

Nature immunology

Allen CD, Ansel KM, Low C, Lesley R, Tamamura H, Fujii N, Cyster JG

An epigenetic view of helper T cell differentiation.

Nature immunology

Ansel KM, Lee DU, Rao A

Overlapping roles of CXCL13, interleukin 7 receptor alpha, and CCR7 ligands in lymph node development.

The Journal of experimental medicine

Luther SA, Ansel KM, Cyster JG

Traffic patterns of B cells and plasma cells.

Advances in experimental medicine and biology

Cyster JG, Ansel KM, Ngo VN, Hargreaves DC, Lu TT

Chemokines in lymphopoiesis and lymphoid organ development.

Current opinion in immunology

Ansel KM, Cyster JG

Follicular stromal cells and lymphocyte homing to follicles.

Immunological reviews

Cyster JG, Ansel KM, Reif K, Ekland EH, Hyman PL, Tang HL, Luther SA, Ngo VN

A chemokine-driven positive feedback loop organizes lymphoid follicles.

Nature

Ansel KM, Ngo VN, Hyman PL, Luther SA, Förster R, Sedgwick JD, Browning JL, Lipp M, Cyster JG

In vivo-activated CD4 T cells upregulate CXC chemokine receptor 5 and reprogram their response to lymphoid chemokines.

The Journal of experimental medicine

Ansel KM, McHeyzer-Williams LJ, Ngo VN, McHeyzer-Williams MG, Cyster JG

Chemokines and B-cell homing to follicles.

Current topics in microbiology and immunology

Cyster JG, Ngo VN, Ekland EH, Gunn MD, Sedgwick JD, Ansel KM

A B-cell-homing chemokine made in lymphoid follicles activates Burkitt's lymphoma receptor-1.

Nature

Gunn MD, Ngo VN, Ansel KM, Ekland EH, Cyster JG, Williams LT